Journal: Molecular neurobiology

Article Title: CD38 Coordinates with NF-κB to Promote Cochlear Inflammation in Noise-Induced Hearing Loss: the Protective Effect of Apigenin.

doi: 10.1007/s12035-024-04675-7

Figure Lengend Snippet: Fig. 9 CD38 coordinates with NF-κB to promote cochlear inflammation in NIHL. As the closest immune cell to organ of Corti, activated macrophages can express CD38 and consume NAD in organ of Corti, causing NAD + metabolism dysfunction and ultimately leads to cochlear inflammation. In this process, inflammatory factors such as IL-1, IL-6, and TNF-α pro- moted the activation of NF-κB signaling pathway, forming a positive feedback cycle

Article Snippet: Immunofluorescence The cochlear basilar membrane was prepared with the surface preparation method, antibodies used in this experiment were rabbit anti-NF-κB p65 polyclonal antibody (Abcam, ab32536), rat anti-F4/80 polyclonal antibody (Abcam, ab6640), and mouse anti-CD38 monoclonal antibody (Proteintech, 60,006–1-lg).

Techniques: Activation Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Lysosomal cholesterol accumulation in macrophages leading to coronary atherosclerosis in CD 38 −/− mice

doi: 10.1111/jcmm.12788

Figure Lengend Snippet: Lysosomal lipid accumulation attenuates lysosomal lumen acidity. In situ ratiometric results of lysosomal lumen pH from both wild type and CD38 −/− macrophages (* P < 0.05 CD38 −/− versus wild type within the same oxLDL concentrations, n = 5).

Article Snippet: The reagents and analysis kits are commercial products as following: lysosome enrichment kit, cholesterol quantitation kit, nicotinamide, PPADS and BAPTA‐AM (Sigma‐Aldrich; St. Louis, MO, USA); Bodipy 493/503, Alexa Fluor‐594 chicken anti‐rat IgG (Life Technologies; Grand Island, NY, USA); 4‐methylumbelliferyl palmitate, NED‐19, CD38 goat polyclonal antibody and lysosome‐associated membrane protein 1 (LAMP‐1) rat monoclonal antibody (Santa Cruz Biotechnology, Inc. Dallas, TX, USA); mouse full‐length CD38 constructs (accession number: NM_007646.2 ), CD38 siRNA (OriGene Technologies, Inc.; Rockville, MD, USA); GenMute and GenJet (SignaGen Laboratories; Rockville, MD, USA), oxLDL (oxidized low‐density lipoprotein) and Dil‐oxLDL [1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethylin dicarbocyanine (Dil)‐labelled oxLDL] (Alfa Aesar; Ward Hill, MA, USA); rabbit antimouse CD68 antibody (Bioss Inc.; Woburn, MA, USA); and oil red O staining kit (American Mastertech Scientific; Lodi, CA, USA).

Techniques: In Situ